منابع مشابه
In-solution hybrid capture of bisulfite-converted DNA for targeted bisulfite sequencing of 174 ADME genes
DNA methylation is one of the most important epigenetic alterations involved in the control of gene expression. Bisulfite sequencing of genomic DNA is currently the only method to study DNA methylation patterns at single-nucleotide resolution. Hence, next-generation sequencing of bisulfite-converted DNA is the method of choice to investigate DNA methylation profiles at the genome-wide scale. Ne...
متن کاملWhole-genome bisulfite DNA sequencing of a DNMT3B mutant patient
The immunodeficiency, centromere instability and facial anomalies (ICF) syndrome is associated to mutations of the DNA methyl-transferase DNMT3B, resulting in a reduction of enzyme activity. Aberrant expression of immune system genes and hypomethylation of pericentromeric regions accompanied by chromosomal instability were determined as alterations driving the disease phenotype. However, so far...
متن کاملWhole-Genome Bisulfite Sequencing
Next-generation sequencing technology has enabled genome-wide analysis of 5mC nucleotides at single-nucleotide resolution. Previous methods for whole-genome bisulfite sequencing (WGBS) yielded reduced genomic representation due to required DNA shearing, ligation of methylated sequencing adapters, and bisulfite conversion of unmethylated cytidine residues prior to sequencing. One disadvantage of...
متن کاملMaternal plasma bisulfite DNA sequencing: tomorrow starts today.
The report in this issue of Clinical Chemistry by Lun, Chiu, and coworkers of the Centre for Research into Circulating Fetal Nucleic Acids at the Chinese University of Hong Kong is an excellent example of this process (2 ). The investigators used genomewide bisulfite DNA sequencing of maternal plasma to comprehensively assess the epigenomic profiles of both fetal and maternal epigenomes noninva...
متن کاملProfiling DNA Methylation Using Bisulfite Sequencing (BS-Seq).
DNA cytosine methylation is one of the most abundant epigenetic marks found in the plant nuclear genome. Bisulfite sequencing (BS-Seq) is the method of choice for profiling DNA cytosine methylation genome-wide at a single nucleotide resolution. The basis of this technique is that the unmethylated cytosine can be deaminated to uracil by sodium bisulfite, while the methylated cytosine is resistan...
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ژورنال
عنوان ژورنال: Current Protocols in Molecular Biology
سال: 2010
ISSN: 1934-3639,1934-3647
DOI: 10.1002/0471142727.mb0709s91